• Corpus ID: 11742677

A sensitive method for determination of cytochrome P4502D6 activity in vitro using bupranolol as substrate.

@article{Appanna1996ASM,
  title={A sensitive method for determination of cytochrome P4502D6 activity in vitro using bupranolol as substrate.},
  author={G Appanna and B. K. Tang and Rudi Mller and Werner Kalow},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={1996},
  volume={24 3},
  pages={
          303-6
        }
}
  • G. Appanna, B. Tang, +1 author W. Kalow
  • Published 1 March 1996
  • Chemistry, Medicine
  • Drug metabolism and disposition: the biological fate of chemicals
Previous studies have shown that bupranolol, a beta-adrenoceptor blocker, is a substrate of cytochrome P4502D6 (CYP2D6). A sensitive in vitro assay was developed to quantify the formation of hydroxybupranolol using HPLC. A TLC method, using radiolabeled bupranolol, was also developed to test the reproducibility of the two methods. Both of them gave virtually identical results; however, the HPLC method was sensitive to 20 pmol and the TLC with radiolabeled substrate to <1 pmol of… 
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