A sensitive immunochemical assay for acetaminophen-protein adducts.

  title={A sensitive immunochemical assay for acetaminophen-protein adducts.},
  author={Dean W Roberts and Neil R Pumford and Douglas W Potter and Ryan W. Benson and Jack A. Hinson},
  journal={The Journal of pharmacology and experimental therapeutics},
  volume={241 2},
The hepatotoxicity of acetaminophen may be mediated by the reactive metabolite N-acetyl-p-benzoquinone imine which binds covalently to protein primarily as 3-(cystein-S-yl)acetaminophen. We have developed an avidin biotin-amplified competitive enzyme-linked immunosorbent assay to detect protein-bound acetaminophen. This assay utilizes antisera from rabbits immunized with 3-(N-acetyl-L-cystein-S-yl)acetaminophen coupled via the carboxyl group to primary amino groups on the protein keyhole-limpet… CONTINUE READING


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