A sensitive fluorometric enzyme-linked immunosorbent assay that measures vascular endothelial growth factor165 in human plasma.

@article{Rodrguez1998ASF,
  title={A sensitive fluorometric enzyme-linked immunosorbent assay that measures vascular endothelial growth factor165 in human plasma.},
  author={Carmen Rueda Rodr{\'i}guez and David T W Fei and Bruce A. Keyt and Deborah L. Baly},
  journal={Journal of immunological methods},
  year={1998},
  volume={219 1-2},
  pages={45-55}
}
A specific and sensitive fluorometric enzyme-linked immunosorbent assay (ELISA) was developed to measure endogenous levels of vascular endothelial growth factor (VEGF165) in human plasma. The ELISA can be performed in 10% human EDTA plasma, yielding a neat plasma sensitivity of 10 pg/ml or 0.2 pM. The recovery of recombinant human VEGF (rhVEGF) added to human plasma ranges from 89 to 100%. The capture antibody depletes the endogenous signal in normal human plasma, suggesting that the signal is… CONTINUE READING

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