A sensitive fluorometric assay for the simultaneous estimation of pepsin and pepsinogen in gastric mucosa.

@article{Ford1982ASF,
  title={A sensitive fluorometric assay for the simultaneous estimation of pepsin and pepsinogen in gastric mucosa.},
  author={T. Foster Ford and John Hermon-Taylor and David A. W. Grant},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1982},
  volume={126 1},
  pages={
          17-23
        }
}
An assay for pepsin has been developed based on the fluorometric measurement of trichloroacetic acid-soluble peptides released from casein at pH 5.3. The increase in relative fluorescence was most sensitive in the range 10-50 micrograms pepsin/l and casein hydrolysis was not affected by the addition of up to a 1000-fold molar excess of pepsinogen. This assay has been used to measure the free and total acid proteinase content of biopsies (less than 5 mg) from different areas of the gastric… CONTINUE READING

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