A sensitive enzyme immunoassay for human basic fibroblast growth factor.

@article{Watanabe1991ASE,
  title={A sensitive enzyme immunoassay for human basic fibroblast growth factor.},
  author={Hiroshi Watanabe and Akira Hori and M. Seno and Yoshio Kozai and Kazuei Igarashi and Yuzo Ichimori and Keiji Kondo},
  journal={Biochemical and biophysical research communications},
  year={1991},
  volume={175 1},
  pages={229-35}
}
A sensitive sandwich enzyme immunoassay for human basic fibroblast growth factor (HbFGF) was developed employing three monoclonal antibodies (MAb3H3, MAb98 and MAb52). The Fab' fragment of MAb3H3 which inhibits HbFGF biological activity was conjugated to horseradish peroxidase. A mixture of MAb52 and MAb98 was used in the solid phase. Neither human acidic fibroblast growth factor, hst-1/KS3 product nor acid denatured HbFGF was cross-reactive in this assay system. The detection limit of this… CONTINUE READING