A sensitive enzymatic method for determination of free and esterified tissue cholesterol.

@article{Carlson1977ASE,
  title={A sensitive enzymatic method for determination of free and esterified tissue cholesterol.},
  author={Susan E. Carlson and Stanley Goldfarb},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1977},
  volume={79 3},
  pages={575-82}
}
An enzymatic assay currently in use in clinical laboratories for quantitating total serum cholesterol has been modified for the determination of microgram quantities of tissue cholesterol. Two types of assays were developed. In the first, more generally useful method, lipids extracted with chloroform/methanol were solubilized by the addition of detergent and assayed for free and total cholesterol using laboratory prepared mixtures of reagents. Although different detergents were effective, it… CONTINUE READING

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