A sensitive dual-fluorescence reporter system enables positive selection of ras suppressors by suppression of ras-induced apoptosis

@article{Dolnikov2003ASD,
  title={A sensitive dual-fluorescence reporter system enables positive selection of ras suppressors by suppression of ras-induced apoptosis},
  author={Alla Dolnikov and Sylvie Shen and Michelle Millington and Toby Passioura and Michelle G. Pedler and John Edward Joshua Rasko and Geoff Symonds},
  journal={Cancer Gene Therapy},
  year={2003},
  volume={10},
  pages={745-754}
}
We have developed a novel dual-fluorescence reporter system incorporating green (GFP) and red (RFP) fluorescent proteins to monitor expression of the N-rasm gene and an N-rasm suppressor, respectively. Retroviral vectors were produced in which human N-rasm (codon 13 mutation) was coexpressed with GFP, and a ribozyme specifically targeting N-rasm was coexpressed with RFP. N-Rasm suppression was monitored by measurement of GFP fluorescence in dual-fluorescent (GFP and RFP) cells. We demonstrated… CONTINUE READING

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