A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles.

  title={A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles.},
  author={Wangxin Xue and Guanxin Zhang and Deqing Zhang},
  journal={The Analyst},
  volume={136 15},
Gold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg(6) due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg(6) with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL(-1) can be assayed with this new colorimetric assay. This colorimetric label… 

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