A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase

@article{Kalimuthu2014ASA,
  title={A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase},
  author={Palraj Kalimuthu and Katrin Fischer-Schrader and Guenter Schwarz and Paul V Bernhardt},
  journal={JBIC Journal of Biological Inorganic Chemistry},
  year={2014},
  volume={20},
  pages={385-393}
}
Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH. The anion anthraquinone-2-sulfonate (AQ) is used as an effective artificial electron transfer partner for NR at a glassy carbon (GC) electrode. Nitrate is enzymatically reduced to nitrite and the oxidized form of NR is electrochemically reduced by the hydroquinone form of the mediator (AQH2). The GC/NR electrode shows a… CONTINUE READING

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