A sensitive and specific method for measurement of multiple retinoids in human serum with UHPLC-MS/MS.

@article{Arnold2012ASA,
  title={A sensitive and specific method for measurement of multiple retinoids in human serum with UHPLC-MS/MS.},
  author={Samuel Arnold and John K Amory and Thomas J Walsh and Nina Isoherranen},
  journal={Journal of lipid research},
  year={2012},
  volume={53 3},
  pages={
          587-98
        }
}
Retinol (vitamin A) circulates at 1-4 μM concentration and is easily measured in serum. However, retinol is biologically inactive. Its metabolite, retinoic acid (RA), is believed to be responsible for biological effects of vitamin A, and hence the measurement of retinol concentrations is of limited value. A UHPLC-MS/MS method using isotope-labeled internal standards was developed and validated for quantitative analysis of endogenous RA isomers and metabolites. The method was used to measure… CONTINUE READING

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