A sensitive and practical method to detect the T790M mutation in the epidermal growth factor receptor.

@article{Zhao2016ASA,
  title={A sensitive and practical method to detect the T790M mutation in the epidermal growth factor receptor.},
  author={Jing Zhao and Hua-Hua Feng and Jin-Yin Zhao and Li-Cheng Liu and Fei-Fei Xie and Yan Xu and M Q Chen and Wei Zhong and Long-Yun Li and Han-ping Wang and Linqi Zhang and Y I Xiao and Wei-jun Chen and Meng-zhao Wang},
  journal={Oncology letters},
  year={2016},
  volume={11 4},
  pages={2573-2579}
}
The current study aimed to develop a method to rapidly, sensitively and practically screen for the epidermal growth factor receptor (EGFR) T790M mutation. This method combines an allele-specific competitive blocker (ACB) with a TaqMan quantitative polymerase chain reaction (PCR) amplification refractory mutation system (ARMS) in a one-step reaction. Using a mimic of a human genomic DNA panel containing serially diluted mutant alleles, the performance efficacy of this method was assessed. Using… CONTINUE READING