A sensitive, type-specific, fluorogenic probe assay for detection of human papillomavirus DNA.

@article{Swan1997AST,
  title={A sensitive, type-specific, fluorogenic probe assay for detection of human papillomavirus DNA.},
  author={D. L. C. Brinkhorst-van der Swan and Ruth Ann Tucker and Brian P. Holloway and Joseph P. Icenogle},
  journal={Journal of clinical microbiology},
  year={1997},
  volume={35 4},
  pages={886-91}
}
A simple method for the detection of a number of human papillomavirus (HPV) genotypes associated with cervical cancer has been developed. The assay exploits the 5'-->3' exonucleolytic activity of Taq DNA polymerase to increase the signal from fluorescent dyes by releasing them from genotype-specific probes during PCR. The probes are oligonucleotides with a 5' reporter dye (6-carboxyfluorescein), a quencher dye (6-carboxy-tetramethyl-rhodamine), and a phosphate-blocked 3' end. In the intact… CONTINUE READING
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