A sensitive, specific assay for tissue collagenase using telopeptide-free [3H]acetylated collagen.

@article{Dean1985ASS,
  title={A sensitive, specific assay for tissue collagenase using telopeptide-free [3H]acetylated collagen.},
  author={David D. Dean and Jessica Woessner},
  journal={Analytical biochemistry},
  year={1985},
  volume={148 1},
  pages={174-81}
}
Collagenase is assayed by incubation with soluble, telopeptide-free collagen extracted from rat skin and labeled with [2-3H]acetic anhydride. Collagen is cleaved by collagenase and the resulting fragments are digested with trypsin and chymotrypsin. Undigested collagen is recovered by precipitation with trichloroacetic acid, collected on glass-fiber filters, and quantitated by liquid scintillation spectrometry. This procedure combines features of the Cawston and Barrett (T.E. Cawston and A.J… CONTINUE READING

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