A sensitive, robust high-throughput electrochemiluminescence assay for rat insulin.

@article{Golla2004ASR,
  title={A sensitive, robust high-throughput electrochemiluminescence assay for rat insulin.},
  author={Rajasree Golla and Ramakrishna Seethala},
  journal={Journal of biomolecular screening},
  year={2004},
  volume={9 1},
  pages={62-70}
}
In diabetes research, mouse and rat models are used for in vivo experiments, and quantification of insulin in serum samples under different pathophysiological conditions and after treatment with compounds is essential. There are few commercial radioimmunoassay and enzyme-linked immunosorbent assay (ELISA) assay kits to determine the rat/mouse plasma levels of insulin. However, reliability in insulin measurements using the available biological assays is a great concern. The authors report a… CONTINUE READING