A semiquantitative method for the determination of reactive metabolite conjugate levels in vitro utilizing liquid chromatography-tandem mass spectrometry and novel quaternary ammonium glutathione analogues.

@article{Soglia2006ASM,
  title={A semiquantitative method for the determination of reactive metabolite conjugate levels in vitro utilizing liquid chromatography-tandem mass spectrometry and novel quaternary ammonium glutathione analogues.},
  author={John R Soglia and Leonard G Contillo and Amit S Kalgutkar and Sabrina X Zhao and Cornelis E. C. A. Hop and James G. Boyd and Mark J Cole},
  journal={Chemical research in toxicology},
  year={2006},
  volume={19 3},
  pages={480-90}
}
An in vitro semiquantitative reactive metabolite detection assay is described that incorporates NADPH-supplemented human liver microsomes, a novel quaternary ammonium glutathione analogue conjugating agent (QA-GSH), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for detection. The assay was developed to have high sample capacity and the potential for high sample throughput. MS/MS detection is selective and sensitive for the QA-GSH conjugating agent and semiquantitation of QA-GSH… CONTINUE READING