A semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process.

@article{Aumann2008AS3,
  title={A semicontinuous 3-column countercurrent solvent gradient purification (MCSGP) process.},
  author={Lars Aumann and Massimo Morbidelli},
  journal={Biotechnology and bioengineering},
  year={2008},
  volume={99 3},
  pages={728-33}
}
The recently developed continuous Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) Process has been reduced to a fully equivalent semicontinuous setup with only three chromatographic columns and three gradient pump modules. Actually the 3-column MCSGP unit can even achieve better performance than the original 6-column process due to an additional degree of freedom, that is a different switching time for the "batch lane" and the "interconnected lane." Experimental results for the… CONTINUE READING