A semi-automated and highly sensitive streptavidin magnetic capture-hybridization RT-PCR assay: application to genus-wide or species-specific detection of several viruses of ornamental bulb crops.

Abstract

A semi-automated, rapid and sensitive method that combines magnetic capture-hybridization and reverse-transcription polymerase chain reaction (MCH/RT-PCR) for the detection of plant viruses is described. The assay uses a target specific biotin-labelled oligoprobe for RNA capture and streptavidin-coated magnetic beads for subsequent RNA-oligoprobe hybrid… (More)

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@article{Miglino2007ASA, title={A semi-automated and highly sensitive streptavidin magnetic capture-hybridization RT-PCR assay: application to genus-wide or species-specific detection of several viruses of ornamental bulb crops.}, author={Roberto Miglino and Agata Jodlowska and Hanu R. Pappu and Ton R van Schadewijk}, journal={Journal of virological methods}, year={2007}, volume={146 1-2}, pages={155-64} }