A selective protein sensor for heparin detection.

  title={A selective protein sensor for heparin detection.},
  author={Shenshen Cai and Jodi L Dufner-Beattie and Glenn D. Prestwich},
  journal={Analytical biochemistry},
  volume={326 1},
No clinical assays for the direct detection of heparin in blood exist. To create a heparin sensor, the hyaluronan (HA)-binding domain (HABD) of a protein that binds heparin and HA was engineered. GST fusion proteins containing one to three HABD modules were cloned, expressed, and purified. The affinities of each construct for heparin and for HA were determined by a competitive enzyme-linked immunosorbent assay using immobilized HA or heparin. Each of the constructs showed modest affinity for… CONTINUE READING