A selective isolation procedure for Micromonospora.


A selective medium containing 25 to 50 micrograms per ml of tunicamycin was devised to isolate micromonosporae from soil samples, making possible simple, preferential isolation of a variety of Micromonospora. When a large amount of Gram-negative bacteria was present in a sample, alkaline treatment (0.01 N NaOH, 5 approximately 10 minutes at 15 degrees C) wa… (More)


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