A segmented gas/liquid delivery system for continuous culture of microorganisms on insoluble substrates and its use for growth of Ruminococcus flavefaciens on cellulose

@article{Weimer2004ASG,
  title={A segmented gas/liquid delivery system for continuous culture of microorganisms on insoluble substrates and its use for growth of Ruminococcus flavefaciens on cellulose},
  author={Paul J. Weimer and Yan Shi and Christine L. Odt},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={36},
  pages={178-183}
}
An anaerobic continuous culture device was constructed that permits accurate delivery of media containing insoluble substrates, even at very low volumetric flow rates (<3 ml/h). The system consisted of: (1) a reservoir in which the medium slurry was mixed well by the combined use of stirring and diffusive gas sparging to suspend a cellulose substrate of small (< 45 μm) particle size; (2) a delivery system that segmented the slurry into small (~ 20μl) discrete liquid segments separated by… CONTINUE READING