A second-derivate fitting algorithm for the quantification of free hemoglobin in human plasma.

@article{Paal2018ASF,
  title={A second-derivate fitting algorithm for the quantification of free hemoglobin in human plasma.},
  author={Michael Paal and Alexander Lang and Georg Hennig and Marie-Luise Buchholtz and Ronald Sroka and Michael Vogeser},
  journal={Clinical biochemistry},
  year={2018},
  volume={56},
  pages={
          62-69
        }
}
BACKGROUND Assessment of hemolysis in vivo is becoming increasingly relevant in critical care. Current methods (Harboe, 1959) for quantifying the free hemoglobin (fHb) content produce unsatisfactory results in case of hyperbilirubinemia, a frequent condition in patients at risk for intravascular hemolysis. METHODS A novel evaluation method based on second-derivative fitting to quantify fHb content was developed. The method uses spectrophotometric data from 350 to 650 nm recorded with standard… CONTINUE READING
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