A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction.

@article{Linster2008ASG,
  title={A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction.},
  author={Carole L. Linster and Lital N. Adler and Kristofor J. Webb and Kathryn C Christensen and Charles Brenner and Steven G. Clarke},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 27},
  pages={18483-92}
}
The Arabidopsis thaliana VTC2 gene encodes an enzyme that catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in the first committed step of the Smirnoff-Wheeler pathway to plant vitamin C synthesis. Mutations in VTC2 had previously been found to lead to only partial vitamin C deficiency. Here we show that the Arabidopsis gene At5g55120… CONTINUE READING