A search for site-filling ligands in the Mcg Bence-Jones dimer: crystal binding studies of fluorescent compounds.

@article{Edmundson1984ASF,
  title={A search for site-filling ligands in the Mcg Bence-Jones dimer: crystal binding studies of fluorescent compounds.},
  author={Allen B. Edmundson and Kathryn R. Ely and James N. Herron},
  journal={Molecular immunology},
  year={1984},
  volume={21 7},
  pages={561-76}
}
In trigonal crystals grown in 1.9 M ammonium sulfate buffered at pH 6.2, the Mcg light-chain (Bence-Jones) dimer has a highly aromatic binding cavity accessible to a wide range of hydrophobic and aromatic ligands. A search was made for site-filling ligands by diffusing compounds into the crystals and determining their locations, orientations and relative occupancies by difference Fourier analysis at 2.7-A resolution. 1-Anilinonaphthalene-8-sulfonate, a small ligand in comparison with the rest… CONTINUE READING