A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects

Abstract

Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (position 2-8 of either siRNA strand counting… (More)
DOI: 10.1093/nar/gkq341

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Cite this paper

@inproceedings{Bramsen2010ASO, title={A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects}, author={Jesper Bertram Bramsen and Malgorzata Maria Pakula and Thomas B Hansen and Claus Bus and Niels Langkj{\ae}r and Dalibor Odadzic and Romualdas Smicius and Suzy L. Wengel and Jyoti Chattopadhyaya and Joachim W. Engels and Piet Herdewijn and Jesper Wengel and J\orgen Kjems}, booktitle={Nucleic acids research}, year={2010} }