A scanning confocal microscope for transmission and reflection imaging

@article{Dixon1991ASC,
  title={A scanning confocal microscope for transmission and reflection imaging},
  author={Adam Dixon and Savvas Damaskinos and Mariette R. Atkinson},
  journal={Nature},
  year={1991},
  volume={351},
  pages={551-553}
}
IN a confocal scanning laser microscope1,2 (CSLM), the detector pinhole is confocal with the illuminated spot on the sample, and rejects light reflected from objects that are not in the focal plane. This results in images that contain only sharp or empty areas, as opposed to non-confocal images which contain sharp and blurry areas, and allows the CSLM to perform optical sectioning. Individual optical sections can be used to produce a true three-dimensional image or can be added together to… CONTINUE READING

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