A sandwich hybridization assay employing enzyme amplification for termination of specific ribosomal RNA from unpurified cell lysates.

@article{Wicks1998ASH,
  title={A sandwich hybridization assay employing enzyme amplification for termination of specific ribosomal RNA from unpurified cell lysates.},
  author={Bryan S. Wicks and David B. Cook and Michael R. Barer and Anthony G O'donnell and Colin H. Self},
  journal={Analytical biochemistry},
  year={1998},
  volume={259 2},
  pages={258-64}
}
We have employed the power of the cyclic NAD-based enzyme amplification system to the determination of 16S rRNA. This generally applicable system employs two oligonucleotide probes, one of which is captured on a microtiter well surface and the other labeled with alkaline phosphatase. The detection of very low levels of hybridization of the capture probe is then achieved by the means of the ultrasensitive enzyme-amplified assay system, resulting in a highly sensitive, convenient, and rapid… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…