A role for heterologous gap junctions between melanoma and endothelial cells in metastasis.

@article{Ito2000ARF,
  title={A role for heterologous gap junctions between melanoma and endothelial cells in metastasis.},
  author={Akiko Ito and Fumitaka Katoh and Tatsuki R Kataoka and Morihito Okada and Nobuyuki Tsubota and Hideo Asada and Kazuhiro Yoshikawa and Sakan Maeda and Yukari Kitamura and Hisato Yamasaki and Hiroshi Nojima},
  journal={The Journal of clinical investigation},
  year={2000},
  volume={105 9},
  pages={1189-97}
}
F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. We found that connexin (Cx) 26 is upregulated in BL6 cells. To examine gap junction formation, we devised a coculture system, in which an opened vein segment was placed at the bottom of a culture dish and then dye-labeled melanoma cells were seeded onto it. Immunohistochemistry indicated that the vein segment preserved the integrity of the… CONTINUE READING