A role for dNTP binding of human immunodeficiency virus type 1 reverse transcriptase in viral mutagenesis.

@article{Weiss2004ARF,
  title={A role for dNTP binding of human immunodeficiency virus type 1 reverse transcriptase in viral mutagenesis.},
  author={Kellie K Weiss and Renxiang Chen and Mark A. Skasko and Holly M. Reynolds and Kwi Yeon Lee and Robert A Bambara and Louis M Mansky and Baek ki Kim},
  journal={Biochemistry},
  year={2004},
  volume={43 15},
  pages={4490-500}
}
HIV-1 reverse transcriptase (RT) is a highly error prone DNA polymerase. We assessed whether the ability of RT to bind nucleotide substrates affects viral mutagenesis. Structural modeling predicts that the V148 and Q151 residues influence the interaction between RT and the incoming dNTP. When we introduce either a V148I or Q151N mutation, RT fidelity increases 8.7- or 13-fold, respectively, as measured by the M13 lacZalpha forward mutation assay. Interestingly, pre-steady state kinetic studies… CONTINUE READING