A robust method for detecting CHK2/RAD53 mutations in genomic DNA.

@article{Sodha2002ARM,
  title={A robust method for detecting CHK2/RAD53 mutations in genomic DNA.},
  author={Nayanta Sodha and Richard S. Houlston and Richard T. Williams and Martin A.R. Yuille and John Mangion and Rosalind A. Eeles},
  journal={Human mutation},
  year={2002},
  volume={19 2},
  pages={173-7}
}
While screening for germline CHK2 mutations in cancer cases by heteroduplex CSGE, we observed that additional PCR fragments were generated from the 3' end region of the gene that includes exons 11-14. Direct sequencing of these fragments suggested that homologous loci (possibly pseudogenes) were concomitantly being amplified. Searches of public sequence databases showed that a number of areas of the genome show a high degree of homology to exons 10-14 of the CHK2 gene. The presence of these… CONTINUE READING