A robust diffusion-based gradient generator for dynamic cell assays.

@article{Atencia2012ARD,
  title={A robust diffusion-based gradient generator for dynamic cell assays.},
  author={Javier Atencia and Gregory Cooksey and Laurie E. Locascio},
  journal={Lab on a chip},
  year={2012},
  volume={12 2},
  pages={309-16}
}
This manuscript describes a new method to generate purely diffusive chemical gradients that can be modified in time. The device is simple in its design and easy to use, which makes it amenable to study biological processes that involve static or dynamic chemical gradients such as chemotaxis. We describe the theory underlying the convection-free gradient generator, illustrate the design to implement the theory, and present a protocol to align multiple layers of double sided tape and laminates to… CONTINUE READING