A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

@article{Derzelle2006ARD,
  title={A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae},
  author={Sylviane Derzelle and Françoise Dilasser},
  journal={BMC Microbiology},
  year={2006},
  volume={6},
  pages={100 - 100}
}
Enterobacter sakazakii is the causative agent of rare but severe food-borne infections associated with meningitis, necrotizing enterocolitis and sepsis in infants. Rehydrated powdered infant formulae have been implicated as the source of infection in several outbreaks and sporadic cases. In this work, a real time fluorescence resonance energy transfer PCR assay incorporating an internal amplification control (IAC) was developed for the specific detection of E. sakazakii in foods. Performance of… CONTINUE READING