A roadmap to directed enzyme evolution and screening systems for biotechnological applications.

@article{Martnez2013ART,
  title={A roadmap to directed enzyme evolution and screening systems for biotechnological applications.},
  author={R. Mart{\'i}nez and U. Schwaneberg},
  journal={Biological research},
  year={2013},
  volume={46 4},
  pages={
          395-405
        }
}
  • R. Martínez, U. Schwaneberg
  • Published 2013
  • Computer Science, Medicine
  • Biological research
  • Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein… CONTINUE READING
    39 Citations

    Figures, Tables, and Topics from this paper

    Explore Further: Topics Discussed in This Paper

    Machine learning-assisted enzyme engineering.
    • 1
    Directed evolution 2.0: improving and deciphering enzyme properties.
    • 66
    • PDF
    Engineering Novel and Improved Biocatalysts by Cell Surface Display.
    • 35
    • PDF
    Deletion and Randomization of Structurally Variable Regions in B. subtilis Lipase A (BSLA) Alter Its Stability and Hydrolytic Performance Against Long Chain Fatty Acid Esters
    • 1
    • PDF
    Reporter Immobilization Assay (REIA) for Bioconjugating Reactions.
    • 3
    High‐throughput droplet‐based microfluidics for directed evolution of enzymes
    • 7

    References

    SHOWING 1-10 OF 73 REFERENCES
    Directed evolution: tailoring biocatalysts for industrial applications
    • 80
    Improving Biocatalyst Performance by Integrating Statistical Methods into Protein Engineering
    • 26
    • PDF
    The diversity challenge in directed protein evolution.
    • 109
    Directed evolution of oxygenases: screening systems, success stories and challenges.
    • 81
    To get what we aim for – progress in diversity generation methods
    • 59
    Directed enzyme evolution.
    • 278
    • PDF
    Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst.
    • 176
    An overview on fermentation, downstream processing and properties of microbial alkaline proteases
    • 365