A ring of negative charges in the intracellular vestibule of Kir2.1 channel modulates K+ permeation.

  title={A ring of negative charges in the intracellular vestibule of Kir2.1 channel modulates K+ permeation.},
  author={H. Chang and Shih-Hao Yeh and R. Shieh},
  journal={Biophysical journal},
  volume={88 1},
  • H. Chang, Shih-Hao Yeh, R. Shieh
  • Published 2005
  • Medicine, Chemistry
  • Biophysical journal
  • The glutamate at site 224 of a Kir2.1 channel plays an important role in K+ permeation. The single-channel inward current flickers with reduced conductance in an E224G mutant. We show that open-channel fluctuations can also be observed in E224C, E224K, and E224Q mutants. Yet, open-channel fluctuations were not observed in either the wild-type or an E224D mutant. Introducing a negatively charged methanethiosulfonate reagent to the E224C mutant irreversibly increased channel conductance and… CONTINUE READING
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