A ring of negative charges in the intracellular vestibule of Kir2.1 channel modulates K+ permeation.

@article{Chang2005ARO,
  title={A ring of negative charges in the intracellular vestibule of Kir2.1 channel modulates K+ permeation.},
  author={H Y Chang and Shih-Hao Yeh and R L Shieh},
  journal={Biophysical journal},
  year={2005},
  volume={88 1},
  pages={243-54}
}
The glutamate at site 224 of a Kir2.1 channel plays an important role in K+ permeation. The single-channel inward current flickers with reduced conductance in an E224G mutant. We show that open-channel fluctuations can also be observed in E224C, E224K, and E224Q mutants. Yet, open-channel fluctuations were not observed in either the wild-type or an E224D mutant. Introducing a negatively charged methanethiosulfonate reagent to the E224C mutant irreversibly increased channel conductance and… CONTINUE READING

From This Paper

Topics from this paper.
13 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

Similar Papers

Loading similar papers…