• DOI:10.1007/s11103-006-9093-z
Corpus ID: 21280525

A rice gene activation/knockout mutant resource for high throughput functional genomics

@article{Hsing2006ARG,
  title={A rice gene activation/knockout mutant resource for high throughput functional genomics},
  author={Yue-Ie Hsing and Chyr-Guan Chern and Ming-Jen Fan and Po-chang Lu and Ku-Ting Chen and Shuen-Fang Lo and Peng-Kai Sun and Shin-Lon Ho and K. Lee and Y. Wang and Wen-Lii Huang and Swee-Suak Ko and S. Chen and Jyh-Long Chen and Chun-I Chung and Yao-Cheng Lin and A. Hour and Yet-Walt Wang and Ya-Chi Chang and Min-Wei Tsai and Yi-Show Lin and Yin-Chin Chen and Hsing-Mu Yen and Charng-Pei Li and Chiu-Kai Wey and Ching-Shan Tseng and Ming-Hsing Lai and Sheng-Chung Huang and L. Chen and S. Yu},
  journal={Plant Molecular Biology},
  year={2006},
  volume={63},
  pages={351-364}
}

Yue-Ie Hsing, Chyr-Guan Chern, +27 authors S. Yu

Published 2006

Biology, Medicine

Plant Molecular Biology

Using transfer DNA (T-DNA) with functions of gene trap and gene knockout and activation tagging, a mutant population containing 55,000 lines was generated. Approximately 81% of this population carries 1–2 T-DNA copies per line, and the retrotransposon Tos17 was mostly inactive in this population during tissue culture. A total of 11,992 flanking sequence tags (FSTs) have been obtained and assigned to the rice genome. T-DNA was preferentially (∼80%) integrated into genic regions. A total of 19… CONTINUE READING

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