A ribozyme gene and an antisense gene are equally effective in conferring resistance to tobacco mosaic virus on transgenic tobacco

@article{Feyter1996ARG,
  title={A ribozyme gene and an antisense gene are equally effective in conferring resistance to tobacco mosaic virus on transgenic tobacco},
  author={Rob de Feyter and Michael Young and Kirsten A. Schroeder and Elizabeth S Dennis and Werner Gerlach},
  journal={Molecular and General Genetics MGG},
  year={1996},
  volume={250},
  pages={329-338}
}
Ribozymes of the hammerhead class can be designed to cleave a target RNA in a sequence-specific manner and can potentially be used to specifically modulate gene activity. We have targeted the tobacco mosaic virus (TMV) genome with a ribozyme containing three catalytic hammerhead domains embedded within a 1 kb antisense RNA. The ribozyme was able to cleave TMV RNA at all three target sites in vitro at 25°C. Transgenic tobacco plants were generated which expressed the ribozyme or the… CONTINUE READING

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