A ribozyme for the aldol reaction.

@article{Fusz2005ARF,
  title={A ribozyme for the aldol reaction.},
  author={Stefan Fusz and Alexander Eisenf{\"u}hr and Seergazhi G. Srivatsan and Alexander Heckel and Michael Famulok},
  journal={Chemistry \& biology},
  year={2005},
  volume={12 8},
  pages={
          941-50
        }
}

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References

SHOWING 1-10 OF 39 REFERENCES

Novel RNA catalysts for the Michael reaction.

A novel ribozyme with ester transferase activity.

Peptide bond formation by in vitro selected ribozymes

This work demonstrates the in vitro selection of ribozymes (196 nucleotides) that perform the same peptidyl transferase reaction as the ribosome: that is, they can join amino acids by a peptide bond.

RNA-catalysed carbon–carbon bond formation

The successful application of in vitro selection is reported to isolate pyridine-modified RNA molecules that catalyse a Diels-Alder cycloaddition, finding that the RNA molecules accelerate the reaction rate by a factor of up to 800 relative to the uncatalysed reaction.

In vitro evolution of a self-alkylatlng ribozyme

RNA enzymes are postulated to have catalysed all chemical reactions in the earliest living cells, and selection for self-biotinylation yields a transfer RNA-like ribozyme that efficiently catalyses carbon–nitrogen bond formation.

Control of gene expression by a natural metabolite-responsive ribozyme

It is demonstrated that ribozyme switches may have functioned as metabolite sensors in primitive organisms, and further suggest that modern cells retain some of these ancient genetic control systems.

An alcohol dehydrogenase ribozyme

An RNA molecule is reported that exhibits activity analogous to that of alcohol dehydrogenase (ADH) and is coupled with a redox relay between NADH and flavin adenine dinucleotide to give a NAD+-regeneration system.

Reduction of an aldehyde by a NADH/Zn2+ -dependent redox active ribozyme.

This study has demonstrated the reversibility of a hydride transfer chemistry catalyzed by the ADH ribozyme, which shares many features with the protein ADHs, e.g., reversibility and NADH/Zn2+ dependence.

Cleavage of an amide bond by a ribozyme

A variant form of a group I ribozyme, optimized by in vitro evolution for its ability to catalyze magnesium-dependent phosphoester transfer reactions involving DNA substrates, also catalyzes the

Efficient Aldolase Catalytic Antibodies That Use the Enamine Mechanism of Natural Enzymes

Antibodies that catalyze the aldol reaction, a basic carbon-carbon bond-forming reaction, have been generated using the ϵ-amino group of Lys to form an enamine with ketone substrates and use this enamine as a nascent carbon nucleophile to attack the second substrate, an aldehyde, to form a new carbon- carbon bond.