A review of tribromoethanol anesthesia for production of genetically engineered mice and rats

  title={A review of tribromoethanol anesthesia for production of genetically engineered mice and rats},
  author={Robert E. Meyer and Richard E. Fish},
  journal={Lab Animal},
Tribromoethanol (TBE) is easy and inexpensive to make in the laboratory from readily available reagents, requires no special equipment for its administration, and is not subject to federal or state drug enforcement agency regulations. Intraperitoneal (i.p.) injection of TBE results in the simple and rapid induction of short-term surgical anesthesia; however, recent adverse reports about the efficacy and safety of TBE make its continued routine use as a rodent anesthetic controversial. The… 

A cyclodextrin formulation to improve use of the anesthetic tribromoethanol (Avertin®)

The improved solubility of TBE with HP-β-CD and the reduced variability in anesthetic response warrants the further investigation of this formulation, while the value of using the anticholinergic atropine in association with TBE for anesthesia is identified.

Kinetics of proinflammatory cytokines after intraperitoneal injection of tribromoethanol and a tribromoethanol/xylazine combination in ICR mice

It is concluded that a combination of TBE 200 mg/kg (1.25%) and xylazine (10mg/kg) is a safe and effective anesthetic for use in animals.

Repeated administration of tribromoethanol in C57BL/6NHsd mice.

Although tribromoethanol did not produce morbidity, mortality, or pathologic changes in treated animals, it is urged caution in use of tribromeethanol in C57BL/6NHsd mice due to its variable anesthetic effectiveness.

Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources

TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice from different sources that exhibited similar overall responses to a single exposure to TBE anesthesia.

Mice anesthesia, analgesia, and care, Part I: anesthetic considerations in preclinical research.

The purpose of this article is to review the existing literature on anesthetic protocols adopted in mice for molecular imaging studies and to report the experience.

Refinement of anesthetic choice in procedures preceding psychopharmacological studies

The results suggest that tribromoethanol and chloral hydrate are improper anesthetics for surgeries that precede behavioral tests related to anxiety, and Isoflurane or thiopental may be suitable for anesthesia before evaluation in animal models predictive of antidepressant or anxiolytic-like effect.

Mouse Anesthesia: The Art and Science

The science of mouse anesthesia together with the art of applying these anesthetic techniques to provide readers with the knowledge needed for successful anesthetic procedures is discussed to discuss the use of possibly oversimplified anesthetic protocols used for mouse procedures and anesthesia.

Nrf2 transcriptional activity in the mouse affects the physiological response to tribromoethanol.



Efficacy of tribromoethanol anesthesia in mice.

The rapid induction and recovery, adequate surgical plane of anesthesia, and lack of complications make this anesthetic effective and simple to use.

Pathologic changes associated with use of tribromoethanol (avertin) in the Sprague Dawley rat.

Little evidence has been gained from controlled studies that substantiate TBE as anacceptable anesthetic agent in the rat, and TBE was given to female Sprague Dawley rats to de-termine whether pathologic changes were induced by i.p.administration of a low dosage, a therapeuticdosage or a high dosage.

Efficacy and safety of stored and newly prepared tribromoethanol in ICR mice.

Because of the variability in anesthetic effectiveness, pathology, and morbidity and mortality associated with the use of TBE, it is recommended that this anesthetic agent is not recommended for use in ICR mice.

Tribromoethanol (Avertin) as an anaesthetic in mice

In the laboratory during the past 10 years more than 5000 mice have been anaesthetized with tribromoethanol, using the methods of Hogan et a1.

Adverse effects of tribromoethanol as used in the production of transgenic mice

Tribromoethanol caused focal to diffuse necrosis primarily of subperitoneal muscle fibres of the abdominal wall, and, occasionally, necrotic changes on the surface of abdominal organs, associated with acute peritoneal inflammation and fibrinous serositis of the abdomen organs.

Tribromoethanol-medetomidine combination provides a safe and reversible anesthetic effect in Sprague-Dawley rats.

This study demonstrates the safe and effective use of tribromoethanol-medetomidine as an anesthetic in the rat.

An evaluation of tribromoethanol (TBE) as an anaesthetic agent in the Mongolian gerbil (Meriones unguiculatus)

Mongolian gerbils injected i.p. with a 1·25% solution of tribromoethanol (TBE) quickly lost the righting reflex and showed good surgical anaesthesia, the duration of which was positively related to

A review of laboratory animal anesthesia with chloral hydrate and chloralose.

Based on a literature review and clinical experience, it is suggested that CH or CS anesthesia should be preceded by administration of barbiturates, opioids, alpha-2 agonists, or phenothiazine tranquilizers.