A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency.

@article{Seiving1992ARA,
  title={A reversed activity staining procedure for detection of an acquired antibody against factor XIII in a girl with factor XIII deficiency.},
  author={Birgitta Seiving and Peter Henriksson and Patric Stenberg and Inga Marie Nilsson},
  journal={British journal of haematology},
  year={1992},
  volume={82 2},
  pages={414-6}
}
A new method for detection of inhibitors against factor XIII is described. Agarose gel electrophoresis with normal platelet poor plasma as a factor XIII source included in the gel was combined with reversed activity staining. The procedure was applied to a 10-year-old girl with factor XIII deficiency where substitution therapy had failed. Plasma samples from the patient inhibited the factor XIII catalysed incorporation of the fluorescent monodansylthiacadaverine into casein at a position… CONTINUE READING