A reverse transcription-PCR assay to distinguish the four genogroups of male-specific (F+) RNA coliphages.

@article{Friedman2009ART,
  title={A reverse transcription-PCR assay to distinguish the four genogroups of male-specific (F+) RNA coliphages.},
  author={Stephanie D. Friedman and Emilie M Cooper and Lisa M. Casanova and Mark D. Sobsey and Fred J. Genthner},
  journal={Journal of virological methods},
  year={2009},
  volume={159 1},
  pages={47-52}
}
Goals of reducing fecal contamination in recreational, drinking, shellfishing and other waters and accurately assessing risk from exposure can best be attained if tools to distinguish between sources of pollution are available. The male-specific RNA coliphage (FRNA) genogroups display a trend of source specificity. Reverse transcription-PCR (RT-PCR) can be effectively used for genotyping if specific primer sets are designed to be capable of identifying all members within each genogroup. In this… CONTINUE READING

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