A retrovirus-based system to stably silence hepatitis B virus genes by RNA interference

@article{Jia2006ARS,
  title={A retrovirus-based system to stably silence hepatitis B virus genes by RNA interference},
  author={Fang Jia and Yi-Zheng Zhang and Chang-Mei Liu},
  journal={Biotechnology Letters},
  year={2006},
  volume={28},
  pages={1679-1685}
}
RNA interference (RNAi) might be an efficient antiviral therapy for some obstinate illness. Herein, a retrovirus-based RNAi system was developed to drive expression and delivery of Hepatitis B virus (HBV)-specific short hairpin RNA (shRNA) in HepG2 cells. The levels of HBsAg and HBeAg and that of HBV mRNA were dramatically decreased by this RNAi system in HepG2 cells transfected with Topo-HBV plasmid. Retrovirus-based RNAi thus may be useful for therapy in HBV and other viral infections and… Expand
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References

SHOWING 1-10 OF 18 REFERENCES
Small interfering RNA inhibits hepatitis B virus replication in mice.
TLDR
Results suggest that siRNA is capable of inhibiting HBV replication in vivo and thus may constitute a new therapeutic strategy for HBV infection. Expand
Stable inhibition of hepatitis B virus expression and replication by expressed siRNA.
  • G. Ren, X. Bai, +6 authors J. Lian
  • Biology, Medicine
  • Biochemical and biophysical research communications
  • 2005
TLDR
The specific siRNA can knock down the HBV gene expression and replication in vitro, and the silence effects have no relationship with interferon response. Expand
Inhibition of hepatitis B virus in mice by RNA interference
TLDR
It is shown that RNAi can be applied to inhibit production of HBV replicative intermediates in cell culture and in immunocompetent and immunodeficient mice transfected with an HBV plasmid, showing that such an approach could be useful in the treatment of viral diseases. Expand
Inhibition of HBV replication by siRNA in a stable HBV‐producing cell line
Potent inhibition of endogenous gene expression by RNA interference has been achieved by using sequence‐specific posttranscriptional gene silencing through the action of small interfering RNAExpand
Inhibition of hepatitis B virus expression and replication by RNA interference
TLDR
A new RNAi system is identified in the RT regions of hepatitis B virus genome, and the corresponding retrovirus vectors, which can remarkably inhibit the replication and expression of HBV, are also constructed. Expand
Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs
TLDR
Whether siRNAs can also silence the expression of the cytoplasmically replicating hepatitis C virus (HCV) RNAs by using a replicon system that supports robust HCV replication, but not the production of infectious virions is investigated. Expand
Induction of an interferon response by RNAi vectors in mammalian cells
TLDR
It is reported here that a substantial number of shRNA vectors can trigger an interferon response and is thought to be too short to induce interferons expression. Expand
RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription
TLDR
It is shown that short interfering RNAs (siRNAs) specific for conserved regions of the viral genome can potently inhibit influenza virus production in both cell lines and embryonated chicken eggs, suggesting that viral mRNA is the target of RNA interference. Expand
Retroviral delivery of small interfering RNA into primary cells
  • G. Barton, R. Medzhitov
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
It is demonstrated that this retroviral system for delivery of siRNAs into cells allows for stable inactivation of genes in primary cells and can greatly expand the types of cells available for RNA interference analysis. Expand
Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference1
TLDR
It is demonstrated that RNAi mediated by 21-bp dsRNA specifically inhibits HIV-1 infection of permanent cell lines and primary CD4+ T cells and derivatized siRNA could be delivered without lipofectin complexing and in the presence of serum. Expand
...
1
2
...