A reproducible two-step culture system for isolated primary mouse ovarian follicles as single functional units.

@article{Lenie2004ART,
  title={A reproducible two-step culture system for isolated primary mouse ovarian follicles as single functional units.},
  author={Sandy Lenie and Rita G Cortvrindt and Tom Adriaenssens and Joseph Smitz},
  journal={Biology of reproduction},
  year={2004},
  volume={71 5},
  pages={
          1730-8
        }
}
A reproducible two-step culture system for isolated mouse ovarian follicles smaller than 100 microm (type 3a follicles) was designed. First, isolated follicles were grown in single droplets of alpha-minimal essential medium (MEM) without (deoxy)ribonucleosides at a lower concentration of fetal bovine serum (FBS; 1%) for 6 days with mechanical prohibition of thecal cell attachment. Growing follicles reaching at least 100 microm were transferred to alpha-MEM medium enriched with a higher… CONTINUE READING
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