A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

@article{Stuchbury1975ARG,
  title={A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.},
  author={Trevor Stuchbury and M. S. Shipton and Robert B. Norris and J. Paul G. Malthouse and Keith Brocklehurst and John A. L. Herbert and Hans Suschitzky},
  journal={The Biochemical journal},
  year={1975},
  volume={151 2},
  pages={
          417-32
        }
}
1. 4-(N-2-Aminoethyl2'-pyridyl disulphide)-7-nitrobenzo-2-oxa-1,3-diazole (compound I) was synthesized and evaluated as a fluorescent labelling reagent for thiol groups. 2. The design of compound (I) as one example of a general type of reporter group delivery reagent (2-pyridyl-S-S-X, where X contains an environmentally sensitive spectroscopic probe) is discussed. 3. The electronic absorption spectrum of compound (I) was determined over a wide range of pH and the spectral changes that accompany… CONTINUE READING
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