A reporter gene assay to assess the molecular mechanisms of xenobiotic-dependent induction of the human CYP3A4 gene in vitro.

@article{Ogg1999ARG,
  title={A reporter gene assay to assess the molecular mechanisms of xenobiotic-dependent induction of the human CYP3A4 gene in vitro.},
  author={Malcolm S. Ogg and Julie Marie Williams and Michael H. Tarbit and Peter S. Goldfarb and Tim J. B. Gray and Gordon G. Gibson},
  journal={Xenobiotica; the fate of foreign compounds in biological systems},
  year={1999},
  volume={29 3},
  pages={
          269-79
        }
}
1. A plasmid containing 1 kb of the CYP3A4 regulatory (promoter) region coupled to a reporter gene for secretary placental alkaline phosphatase (SPAP) was transfected into HepG2 cells. Transfected cells were dosed with several known inducers of CYP3A4 and the levels of SPAP were measured. The effect of co-transfecting a plasmid encoding the human glucocorticoid receptor on reporter gene activity was also examined. 2. Dexamethasone induced CYP3A4-dependent reporter gene expression in a… CONTINUE READING
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