A reliable method for avoiding false negative results with Luminex single antigen beads; evidence of the prozone effect.

@article{Carey2016ARM,
  title={A reliable method for avoiding false negative results with Luminex single antigen beads; evidence of the prozone effect.},
  author={B. Sean Carey and Kim Boswijk and Mazen Mabrok and Peter A. Rowe and Andrew Connor and Imran Saif and Anthony Poles},
  journal={Transplant immunology},
  year={2016},
  volume={37},
  pages={
          23-27
        }
}
Luminex single antigen bead (SAB) assays have become an essential tool in monitoring the status of antibody to the Human Leucocyte Antigen (HLA) of patients both before and after transplantation. In addition SAB data is used to aid risk stratification to assess immunological risk of humoral rejection in solid organ transplantation (CTAG/BTAG guidelines) [1]. Increasingly laboratories are reporting false negative results at high antibody titre due to a prozone effect. Here we report a case study… CONTINUE READING