A reliable enzyme linked immunosorbent assay for the determination of bovine and porcine gelatin in processed foods.

@article{Doi2009ARE,
  title={A reliable enzyme linked immunosorbent assay for the determination of bovine and porcine gelatin in processed foods.},
  author={Hirotoshi Doi and Eriko Watanabe and Haruki Shibata and Soichi Tanabe},
  journal={Journal of agricultural and food chemistry},
  year={2009},
  volume={57 5},
  pages={1721-6}
}
Since gelatin-containing foods pose a risk for eliciting allergic reactions in sensitized individuals, a novel sandwich enzyme linked-immunosorbent assay (ELISA) for the detection and quantification of bovine and porcine gelatin in processed foods was developed. Rabbits and goats were immunized with bovine gelatin, and three antisera (pAb1 and pAb2 from rabbits, and pAb3 from goats) were obtained. We established a sandwich ELISA method based on a combination of these antibodies. In this study… CONTINUE READING
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