A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum

  title={A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum},
  author={Ali Akbar Sharafi and Haleh Hashemi Sohi and A. M. Mousavi and Pejman Azadi and Khadijeh Razavi and Valentine Otang Ntui},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
An efficient and reliable transformation system for a very important medicinal plant Papaver bracteatum was developed through optimization of several factors that affect the rate of effective A. rhizogenes-mediated transformation and growth rate of hairy root. Five bacterial strains, A4, ATCC15834, LBA9402, MSU440 and A13, and three explants types, hypocotyls, leaves and excised shoots were examined. The highest frequency of transformation was achieved using LBA9402 strain in the excised shoots… CONTINUE READING
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