A regioselective tryptophan 5-halogenase is involved in pyrroindomycin biosynthesis in Streptomyces rugosporus LL-42D005.

@article{Zehner2005ART,
  title={A regioselective tryptophan 5-halogenase is involved in pyrroindomycin biosynthesis in Streptomyces rugosporus LL-42D005.},
  author={S. Zehner and A. Kotzsch and Bojan Bister and R. S{\"u}ssmuth and C. M{\'e}ndez and J. Salas and K. van P{\'e}e},
  journal={Chemistry \& biology},
  year={2005},
  volume={12 4},
  pages={
          445-52
        }
}
The antibiotic compound pyrroindomycin B contains an indole ring chlorinated in the 5 position. The indole ring is probably derived from tryptophan, and thus primers derived from conserved regions of tryptophan halogenases were used to amplify and clone a DNA fragment that was then used to isolate a tryptophan 5-halogenase gene (pyrH) from a cosmid library of the pyrroindomycin producer Streptomyces rugosporus LL-42D005. A gene disruption mutant in the tryptophan 5-halogenase gene no longer… Expand
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