A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.

@article{Farmer1992ARA,
  title={A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.},
  author={Bennett T. Farmer and Ronald A. Venters and Leonard D Spicer and Michael Wittekind and Luciano Mueller},
  journal={Journal of biomolecular NMR},
  year={1992},
  volume={2 2},
  pages={195-202}
}
A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated. 

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