A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens

@article{Wang2010ARP,
  title={A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens},
  author={Yu-chun Wang and H. W. Chao and Chia-Hsin Wu and Chu-Han Tseng and Ya-Ting Kuo and Tsui-Chun Tsou},
  journal={Biotechnology Letters},
  year={2010},
  volume={32},
  pages={1789-1796}
}
A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose–response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase… CONTINUE READING